Research

Research Areas at McArdle

Cancer genetics and eEpigenetic Mechanisms pursues basic and translational research to discover novel cancer genetic and epigenetic mechanisms, to translate mechanistic insights derived principally from mouse models and primary human cancer samples to human cancer, and to leverage the mechanistic insights to advance cancer diagnosis and treatment.

Cancer genetics and epigenetics are central to the research of Drs. Christopher Bradfield, Aussie Suzuki, Beth WeaverWei Xu, and Jing Zhang. The scientific basis of these studies arises from the fact that cancer is a genetic disease encompassing both germline and somatic genetics. Current areas of research include:

  • Mouse and rat models to study basic and translational aspects of colon cancer. These efforts include biomarker development as well as advanced imaging applications. A recent advance is an Apc-mutant rat model that enhances the modeling of familial human colon cancer.
  • Mouse models to identify genes predisposing individuals to the risk of developing liver and pancreatic cancer. The liver work focuses on the interaction of genetics and hormonal exposure in the etiology of hepatic carcinoma.
  • Rat models to study the etiology and prevention of breast cancer. These studies integrate genetic studies in the rat with studies of human DNA from epidemiology-linked breast cancer case-control studies. For example, studies on the Rat Mcs5a quantitative trait locus led to the identification of an orthologous human locus associated with breast cancer risk.
  • Mouse models to study environmental xenobiotics.
  • Epigenetic transcriptional control of breast cancer. This work specifically focuses on histone H3 methylation by CARM1 and activation of the estrogen receptor.
  • Mouse models for hematopoietic cancers and the study of genetic changes that alter the balance in growth versus differentiation properties o fhematopoietic stem cells (HSCs) and their progeny lead to these cancers.
  • State of the art microscopy methods and drug studies to define molecular signals and cellular machinery that determine accurate division of cellular chromosomes during mitosis and consequences of mistakes in these processes leading to aneuploidy and its effect on cancer.
  • Defining the role of epigenetic dysregulation in cancer.

Tumor biology includes research groups working on aspects of cell biology, stem cells, transcriptional regulation and protein structure relevant to cell growth regulation, differentiation and cancer.

Tumor biology includes research groups working on aspects of cell biology, stem cells, transcriptional regulation and protein structure relevant to cell growth regulation, differentiation and cancer. McArdle faculty with research interests focused on these areas of tumor biology are: Drs. Christopher Bradfield, Dustin Deming, William Fahl, Shigeki Miyamoto, Yongna Xing, Wei Xu, and Jing Zhang. Current areas of research include:

  • Roles of estrogen receptors, estrogen-related receptors and post-transcriptional mechanisms of estrogen receptor in breast cancer.
  • Oncogenic pathways and modifiers of breast cancer cell behavior including TGFβ, the RNA binding protein CRD-BP, Ras, neu, and Wnt signaling.
  • Structural biology of protein phosphatase 2A and other molecular targets in cancer cells.
  • Roles of hematopoietic stem cells and Ras signaling in mouse models of leukemia.
  • Effects of carcinogen exposure and the aryl hydrocarbon receptor.
  • Development of chemoprotective molecules for alleviating side-effects of chemotherapy.
  • Defining new approaches to targeting estrogen receptor in recurrent, metastatic breast cancer.
  • Lipid metobolism/deposition in the skin and its role in cancer and meta “bolic homeostasis”.
  • Studies of NFkappaB signaling in cancer and immunology.
  • Use of 3-dimensional cultures to test patient’s tumor’s response to targeted therapy– a new pathway to precision medicine.

Human tumor viruses are causally associated with over 15% of all human cancers and are likely to contribute to additional cancers. Recent research in our program and elsewhere clearly demonstrates that, for many virus-associated cancers, viral gene functions are required both for tumor development and also for continued survival of malignant cells. Thus, inhibiting tumor virus infection, replication and maintenance, and/or selected viral oncogenic functions can prevent or cure many of these cancers.

Tumor virology is represented by Drs. Paul Ahlquist, Eric Johannsen, Robert Kalejta, Shannon Kenney, Paul Lambert, Dan Loeb, Nate Sherer, Megan Spurgeon, and Bill Sugden. The strong motivation for this program is that viruses are now known to cause at least 15%-20% of human tumors, and are widely suspected in additional cancers. In many or most of these cases, viral gene functions not only contribute to tumor development, but also are required for continuing survival of the final malignant tumor. Thus, interfering with virus infection or viral functions could prevent or cure many cancers. Current areas of research include:

Epstein-Barr Virus (EBV)
  • Studies on the replication and partitioning of EBV DNAs between dividing cells, EBV control of cell proliferation, and the interaction of EBV viral and cellular microRNAs (miRNAs) with infection and oncogenesis.
  • Studies on EBV latent infection and lytic reactivation, including the roles of viral DNA methylation and host proteins in these processes. Recent work showed that cellular Hsp90 is required for normal expression of EBNA2, an important EBV-transforming protein.
  • Developing treatments for EBV-associated tumors based on reactivation of lytic EBV replication and/or inhibiting EBV functions required for viral maintenance, cell survival and proliferation
Kaposi’s Sarcoma-associated Herpes Virus (KSHV)
  • Studies on viral DNA partitioning required to maintain the latent infections associated with tumorigenicity.
Human Cytomegalovirus (HCMV)
  • Studies on latent and lytic herpesvirus replication. Recent work has shown that HCMV modulates cellular Rb function in part through a viral kinase that acts as a hyperactive ortholog of cellular cyclin-dependent kinases.
Human Papillomaviruses (HPVs)
  • Studies on the pathways and mechanisms of HPV infection, maintenance, and oncogenesis.
  • Specific roles of viral oncogenes, cell genes and hormones in HPV-associated oncogenesis in different tissues. Recent studies show that p53 loss synergizes with estrogen and papillomaviral oncogenes to induce cervical and breast cancers.
  • Fundamental differences in cell cycle deregulation in human papillomavirus-positive and -negative head/neck and cervical cancers.
Hepatitis B virus (HBV)
  • Studies on the molecular processes by which the virus maintains its genome and chronic infections that lead to progressive liver damage and hepatocellular carcinoma.
Hepatitis C virus (HCV)
  • Studies on the molecular processes by which the virus maintains its genome and chronic infections that lead to progressive liver damage and hepatocellular carcinoma.

Ahlquist Lab

We are studying the novel, RNA-based pathways and virus-host interactions underlying replication, gene expression and evolution by positive-strand RNA viruses, the largest class of viruses.

Lab website

Paul Ahlquist

Position title: Principal Investigator

Email: ahlquist@wisc.edu

Phone: (608) 263-5916, (608) 265-9213

Address:
841A Robert M. Bock Laboratories

Megan Bracken

Position title: Senior Research Specialist

Email: mbracken@wisc.edu

Johan den Boon

Position title: Research Investigator

Email: jdenboon@morgridge.org

Amy Freitag

Pronouns: Director's Assistant

Email: apfreitag@wisc.edu

Mark Horswill

Pronouns: Senior Research Specialist

Email: mhorswill@morgridge.org

Helena Jaramillo Mesa

Pronouns: Postdoctoral Research Associate

Email: hjaramillo@morgridge.org

Adam Jochem

Pronouns: Senior Research Specialist

Email: ajochem@morgridge.org

Masaki Nishikiori

Pronouns: Assistant Scientist

Email: mnishikiori@morgridge.org

Dandan Shao

Position title: Postdoc Research Associate

Email: dshao@morgridge.org

Hong Zhan

Pronouns: Associate Research Investigator

Email: hzhan3@wisc.edu

Bradfield Lab

Our laboratory is interested in a family of transcriptional regulators known as PAS proteins.

Christopher Bradfield

Position title: Principal Investigator

Email: bradfield@oncology.wisc.edu

Phone: 608-262-2024

Address:
213A McArdle Laboratory Building

Alex Veith

Position title: Research Associate

Email: acveith@wisc.edu

Churpek Lab

Our lab is focused on defining the contribution of rare genetic variation to risk of blood disorders and cancer.

Lab website

Bianca Barredo

Position title: Clinical Research Coordinator

Email: barredo@wisc.edu

William Blaser

Position title: Honorary Associate

Email: wjblaser@wisc.edu

Jane Churpek

Position title: Principal Investigator

Email: jchurpek@wisc.edu

Address:
600 Highland Ave
Madison WI 53792-3284

Rachel Kirchner

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: rekirchner2@wisc.edu

Dalton Mclean

Position title: Scientist

Email: mclean3@wisc.edu

Sharanya Nath

Position title: Graduate Student

Pronouns: Cancer Biology

Email: snath7@wisc.edu

Sarah Obregon

Position title: Researcher I

Email: saporter2@wisc.edu

Deming Lab

Our laboratory is investigating kinase modulation of cellular signaling pathways driven by oncogenic mutant proteins

Ellie Boeree

Position title: Graduate Student

Pronouns: Cancer Biology

Email: eabybee@wisc.edu

Somak Chaudhuri

Position title: Graduate Student

Pronouns: Cancer Biology

Email: somak.chaudhuri@wisc.edu

Dustin Deming

Position title: Principal Investigator

Email: ddeming@medicine.wisc.edu

Phone: (608) 265-1042

Address:
6507 WI Institute Medical Research

Isoline Donohue

Position title: Graduate Student

Pronouns: Cancer Biology

Email: idonohue@wisc.edu

Katherine Johnson

Pronouns: Research Analyst II

Email: kknudtson@wisc.edu

Cheri Pasch

Position title: Laboratory Manager

Email: capasch@uwcarbone.wisc.edu

Lexie Schmitz

Position title: Graduate Student

Pronouns: Cancer Biology

Email: aeschmitz4@wisc.edu

Ruchi Shah

Position title: Graduate Student

Pronouns: Cancer Biology

Email: rcshah2@wisc.edu

Dinh Lab

Our goal is to understand how tumor immune microenvironment changes toward finding immunotherapeutic biomarkers and targets.

Lab website

Huy Dinh

Position title: Principal Investigator

Email: huy.dinh@wisc.edu

Phone: (608) 263-2890

Address:
1111 Highland Ave
Madison, WI 53705

Anqi Gao

Position title: Graduate Student

Pronouns: Medical Scientist Training Program

Email: agao9@wisc.edu

Parth Khatri

Position title: Graduate Student

Pronouns: Biomedical Data Sciences MS

Email: pkhatri@wisc.edu

Jasjit Mangat

Position title: Research Intern

Email: jmangat@wisc.edu

Sumedha Sanjeev

Position title: Graduate Student

Pronouns: Biomedical Data Sciences MS

Email: srsanjeev@wisc.edu

Stella Tsao

Position title: Graduate Student

Pronouns: Cancer Biology

Email: sttsao@wisc.edu

Johannsen Lab

Our research focuses on the molecular biology of Epstein-Barr virus (EBV) nuclear proteins, their role in the virus lifecycle and the pathogenesis of EBV associated malignancies.

Lab website

Emilie Greene

Position title: Graduate Student

Pronouns: Cancer Biology

Email: egreene4@wisc.edu

Eric Johannsen

Position title: Principal Investigator

Email: ejohannsen@medicine.wisc.edu

Phone: (608) 262-9952

Address:
6531 WI Institute for Medical Research

Makoto Ohashi

Position title: Researcher III

Email: mohashi@medicine.wisc.edu

Mariah Riel

Position title: Graduate Student

Pronouns: Microbiology Doctoral Training Program

Email: mariah.riel@wisc.edu

Lily Wenger

Position title: Graduate Student

Pronouns: Cancer Biology

Email: lcwenger@wisc.edu

Kalejta Lab

Our lab focuses on determining the mechanisms of human cytomegalovirus (HCMV) replication and pathogenesis, and also uses the virus as a tool to probe the pathways that lead to cell cycle progression and oncogenesis.

Lab website

Emily Albright

Position title: Research Scientist

Email: ealbright@wisc.edu

Rob Kalejta

Position title: Principal Investigator

Email: rfkalejta@wisc.edu

Phone: (608) 265-5546, (608) 265-5390

Address:
641 A Robert A. Bock Laboratories

Esther Wisdom

Position title: Graduate Student

Pronouns: Microbiology Doctoral Training Program

Email: ewisdom@wisc.edu

Kenney Lab

Our lab focused upon understanding the molecular regulation and pathogenesis of the human herpesvirus, Epstein-Barr virus (EBV).

Jillian Bristol

Position title: Scientist I

Email: bristol@wisc.edu

Maria Calandranis

Position title: Research Specialist

Email: mcalandranis@wisc.edu

Tony Chen

Position title: Research Intern

Email: zchen842@wisc.edu

Shannon Kenney

Position title: Principal Investigator

Email: skenney@wisc.edu

Phone: (608) 265-0533, (608) 261-1196

Address:
1111 Highland Ave
Madison, WI 53705

Scott Nelson

Position title: Scientist II

Email: senelson4@wisc.edu

Deo Singh

Position title: Scientist II

Email: drsingh@wisc.edu

Chunyan Wang

Position title: Scientist I

Email: cwang723@wisc.edu

Changyan Zhou

Position title: Graduate Student

Pronouns: Cancer Biology

Email: czhou277@wisc.edu

Lambert Lab

Our lab’s research is focused on understanding the role of human papillomavirus (HPV) in cancer.

Dawn Albrecht

Position title: Senior Research Specialist

Email: dmalbrecht@medicine.wisc.edu

Andrea Bilger

Position title: Senior Scientist

Email: bilger@oncology.wisc.edu

Allison Geiger

Position title: Research Intern

Email: ajgeiger4@wisc.edu

Zane Huang

Position title: Research Intern

Email: khuang244@wisc.edu

Donghwan (Heron) Jeon

Position title: Research Associate

Email: donghwan.jeon@wisc.edu

Renee King

Position title: Research Associate

Email: renee.king@wisc.edu

Paul Lambert

Position title: Principal Investigator

Email: lambert@oncology.wisc.edu

Phone: (608) 262-8533, (608) 262-6407

Address:
6459 WI Institutes for Medical Research

Denis Lee

Position title: Researcher III

Email: denis.lee@wisc.edu

Kaitlyn Pham

Position title: Graduate Student

Pronouns: Cancer Biology

Email: lpham22@wisc.edu

Umar Sheikh

Position title: Research Associate

Email: usheikh@wisc.edu

Maggie Stangis

Position title: Graduate Student

Pronouns: Cancer Biology

Email: stangis@wisc.edu

Ella Ward-Shaw

Position title: Research Specialist

Email: etward@wisc.edu

Loeb Lab

We are studying the mechanisms of RNA encapsidation, initiation and synthesis of minus-strand DNA, initiation and synthesis of plus-strand DNA, and genome circularization during plus-strand DNA synthesis.

Stephen Darodda

Position title: Graduate Student

Pronouns: Cellular and Molecular Pathology

Email: darodda@wisc.edu

Dan Loeb

Position title: Principal Investigator

Email: loeb@oncology.wisc.edu

Phone: (608) 262-1260

Address:
6453 WI Institutes for Medical Research

Majumder Lab

We study how viruses localize to cellular sites on the host genome to establish infection by hijacking the cell’s replication and repair machinery.

Lab website

Rhiannon Abrahams

Position title: Graduate Student

Pronouns: Microbiology Doctoral Training Program

Email: rrabrahams@wisc.edu

Clairine Larsen

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: cilarsen@wisc.edu

Kinjal Majumder

Position title: Principal Investigator

Email: kmajumder@wisc.edu

Phone: (608) 262-6386

Address:
741A RM Bock Labs

Sara Maloney, PhD

Position title: Research Associate

Email: smmaloney4@wisc.edu

Gavin Marcoe

Position title: Graduate Student

Pronouns: Cancer Biology

Email: gmarcoe@wisc.edu

Asha Rustad

Position title: Graduate Student

Pronouns: Endocrinology and Reproductive Physiology Training Program

Email: aerustad@wisc.edu

Allison Spillane, PhD

Position title: Research Associate

Email: aklutz@wisc.edu

Ian Stukes

Position title: Research Intern

Email: stukes@wisc.edu

Monnette Summers

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: mfvillarreal@wisc.edu

Miyamoto Lab

Our lab has pursued a variety of research interests centered around cell signaling and asked “what happens when cells are no longer able to communicate effectively?”

Jason Abel

Position title: Professor (CHS)

Email: abel@urology.wisc.edu

Shelly Davis

Position title: Senior Scientist

Email: swuerzbe@wisc.edu

Moon Hee Lee

Position title: Scientist I

Email: mhlee2@wisc.edu

Shigeki Miyamoto

Position title: Principal Investigator

Email: smiyamot@wisc.edu

Phone: (608) 262-9281

Sherer Lab

Our group is interested in the cell biology underlying the assembly and spread of the human immunodeficiency virus type 1 (HIV-1), the etiological agent causing the acquired immunodeficiency syndrome (AIDS).

Lab website

James Bruce

Position title: Senior Staff Virologist

Email: jamesbruce@wisc.edu

Jorge Guerrero, PhD

Position title: Research Associate

Email: jfguerrero2@wisc.edu

Lauren Kelly

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: lkkelly2@wisc.edu

Nate Sherer

Position title: Principal Investigator

Email: nsherer@wisc.edu

Phone: (608) 890-2551

Address:
509A Robert A. Bock Laboratories

Kerry Smith

Position title: Graduate Student

Pronouns: Molecular and Cellular Pharmacology

Email: kasmith35@wisc.edu

Spurgeon Lab

The Spurgeon Lab studies two different small DNA tumor viruses: Merkel cell polyomavirus (MCPyV) and human papillomaviruses (HPVs).

Lab website

Christopher Collins, PhD

Credentials: Spurgeon Lab

Position title: Postdoctoratal Research Associate

Email: ccollins@morgridge.org

Phone: (360) 865-7460

Tejas Sabu

Credentials: Spurgeon Lab

Position title: Graduate Student

Pronouns: Cancer Biology

Email: tsabu@wisc.edu

Chloe Schiff

Credentials: Spurgeon Lab

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: csschiff@wisc.edu

Megan Spurgeon

Position title: Principal Investigator

Email: megan.spurgeon@wisc.edu

Phone: (608) 316-3999

Address:
Morgridge Institute for Research
330 N Orchard St
Madison, WI 53715

Sugden Lab

Our research focuses on two facets of EBV pivotal to its inducing and maintaining human tumors.

Beniah Brumbaugh

Position title: Graduate Student

Pronouns: Cellular and Molecular Pathology

Email: bbrumbaugh@wisc.edu

Madilyn Ellis

Position title: Graduate Student

Pronouns: Cancer Biology

Email: mrellis3@wisc.edu

Isabela Fraga de Andrade, PhD

Position title: Research Associate

Pronouns: Postdoctoral Trainee

Email: fragadeandra@wisc.edu

Phone: (608) 770-4323

Mitch Hayes

Position title: Researcher II

Email: mhayes1@oncology.wisc.edu

Abena Kissi-Twum, PhD

Position title: Research Associate

Email: kissitwum@wisc.edu

Caitlin O'Donnell

Position title: Graduate Student

Pronouns: Bacteriology M.S.

Email: csodonnell@wisc.edu

Bill Sugden

Position title: Principal Investigator

Email: sugden@oncology.wisc.edu

Phone: (608) 262-1116

Address:
6555 WI Institutes for Medical Research

Suzuki Lab

Our lab focuses on discovering the molecular mechanisms underlying force production, mitotic checkpoint control, and error correction in accurate cell division.

Lab website

Yu-Chia Chen

Position title: Graduate Student

Pronouns: Molecular and Cellular Pharmacology

Email: chen988@wisc.edu

Yu-Lin Chen

Position title: Graduate Student

Pronouns: Biophysics

Email: chen2539@wisc.edu

Dhaval Ghone

Position title: Graduate Student

Pronouns: Biophysics

Email: dghone@wisc.edu

Wan-Yi Hsiao

Position title: Research Associate

Email: whsiao6@wisc.edu

Magdalena Lazraj

Position title: Research Intern

Email: lazraj@wisc.edu

Chieh-Chang Lin

Position title: Research Intern

Email: clin445@wisc.edu

Syon Reddy

Position title: Research Intern

Email: smreddy3@wisc.edu

Aussie Suzuki

Position title: Principal Investigator

Email: aussie.suzuki@wisc.edu

Phone: (608) 262-1686

Address:
6459 WI Institute Medical Research

Weaver Lab

Our lab is determining the consequences of mitotic defects on tumor initiation, progression and response to chemotherapy,

Lab website

Mathew Bloomfield

Position title: Research Associate

Email: mbloomfield2@wisc.edu

Caleb Carlsen

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: clcarlsen@wisc.edu

Boya Chen

Position title: Graduate Student

Pronouns: Molecular and Cellular Pharmacology

Email: bchen384@wisc.edu

Christina Firl

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: firl@wisc.edu

Rebeca Garcia-Varela

Position title: Scientist

Email: garciavarela@wisc.edu

Daniel Sam

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: dksam@wisc.edu

Beth Weaver

Position title: Principal Investigator

Email: baweaver@wisc.edu

Phone: (608) 263-5309

Address:
6109 WI Institute of Medical Research

Xing Lab

Our lab studies elucidation of signaling pathways related to cancer using multi-disciplinary biophysics and biochemical approaches, including structural biology and proteomics, in combination with cell biology.

Xueling Ding

Position title: Graduate Student

Pronouns: Comparative Biomedical Sciences

Email: xding73@wisc.edu

Pankaj Singh Parihar

Position title: Research Associate

Email: pparihar@wisc.edu

Yongna Xing

Position title: Principal Investigator

Email: xing@oncology.wisc.edu

Phone: (608) 262-8376, (608) 262-1989

Address:
6451 WI Institute for Medical Research

Xu Lab

Our lab focuses on the transcriptional regulation of estrogen receptor (ER) signaling pathways by nuclear receptor co-factors.

Mia Corradi

Position title: Graduate Student

Pronouns: Molecular and Cellular Pharmacology

Email: mcorradi@wisc.edu

Ang Gao

Position title: Scientist I

Email: agao22@wisc.edu

Ru Jia

Position title: Research Associate

Email: rjia42@wisc.edu

Carstyn Joiner

Position title: Graduate Student

Pronouns: Cancer Biology

Email: cfjoiner@wisc.edu

Gui Ma

Position title: Scientist I

Email: gma24@wisc.edu

Kasey Mitchell

Position title: Graduate Student

Pronouns: Endocrinology and Reproductive Physiology Training Program

Email: kmitchell4@wisc.edu

Yidan Wang

Position title: Research Specialist

Email: wang35@oncology.wisc.edu

Wei Xu

Position title: Principal Investigator

Email: wxu@oncology.wisc.edu

Phone: (608) 265-5540, (608) 262-9834

Address:
7459 WI Institutes for Medical Research

Jingjing Zhou

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: jzhou462@wisc.edu

Zhang Lab

Our lab focuses on studying the mechanisms underlying the normal as well as oncogenic self-renewal of stem cells using the hematopoietic compartment as a model system.

Vysakh Anandan

Position title: Post Doctoral Research Associate

Email: vanandan@wisc.edu

Meher Gayatri Bolisetti

Position title: Research Associate

Email: bolisetti@wisc.edu

Jacqueline Cook

Position title: Graduate Student

Pronouns: Cellular and Molecular Biology

Email: jacook6@wisc.edu

Esra'a Keewan

Pronouns: Research Associate

Email: keewan@wisc.edu

Sophie Verbeke

Position title: Research Intern

Email: sverbeke@wisc.edu

Jing Zhang

Position title: Principal Investigator

Email: zhang@oncology.wisc.edu

Phone: (608) 263-1147, (608) 263-1150

Address:
7453 WI Institutes for Medical Research