UW madison
UW School of Medicine and Public Health
Carbone Cancer Center

Paul F. Lambert, Ph.D.

Paul Lambert
Howard M. Temin Professor and Chair of Oncology
Director - McArdle Laboratory for Cancer Research
Virology Program Leader - UW Carbone Cancer Center

B.S., 1979, Biochemistry, University of Massachusetts, Amherst
Ph.D., 1985, Biochemistry, University of Wisconsin-Madison
Postdoctoral research: Laboratory of Peter Howley, National Cancer Institute

6459 Wisconsin Institutes for Medical Research
Office - (608) 262-8533; Lab - (608) 262-6407
Research Description: 

Our lab’s research is focused on understanding the role of human papillomavirus (HPV) in cancer.  HPVs cause 5% of all human cancers. These include cervical cancer, other anogenital cancers and a growing proportion of head and neck cancers.  Through the use of genetically engineered mice (GEM) we have developed models for HPV-associated cervical, anal and head/neck carcinogenesis, defined the individual roles of viral oncogenes in carcinogenesis in these organs, identified the mechanisms of action by which these oncogenes contribute to carcinogenesis, defined their temporal role in carcinogenesis, and defined the roles of estrogen and its receptor in cervical carcinogenesis and the utility of estrogen receptor antagonists in treating and preventing cervical cancer.  Major current directions in the lab that make continued use of the GEM models include understanding the interplay between viral oncogenes and cellular signaling and DNA damage response pathways in causing cancer, and the influences of viral genes on epigenetic regulation and epithelial stem cell biology.

In recent years we have complemented our use of GEM models with the development patient derived xenografts (PDX) models in which human cancers are directly grafted and passaged in immune-deficient mice. PDX models provide us the ability to test new therapeutic approaches for treating human cancers that we have identified through our studies using the GEM models. For example, using our GEM model for HPV-associated anal cancer, we identified that the cellular mTOR pathway is activated, and its inhibition by rapamycin suppresses anal cancer growth.  We then demonstrated the therapeutic value of rapamycin in treating human anal cancer using the first human anal cancer PDX model. Likewise, working with our clinical colleagues we have now established an array of PDX models for both HPV-positive and HPV-negative human head and neck cancers that will be used alongside our GEM models for these cancers to define novel therapeutic approaches for treating these cancers.

The papillomaviral life cycle is intricately tied to the differentiation of the host epithelium it infects.  Using artificial skin cultures wherein we can recapitulate all the stages of the viral life cycle in differentiating epithelium, we continue our efforts to dissect the role of viral genes and cellular pathways in the viral life cycle.  Recently, we established a mouse papillomavirus infection model in laboratory mice.  This new model will allow us to use the power of mouse genetics to better understand virus-host interactions involved in infection and possibly also virally-induced cancers.

Selected Recent Publications: 


Ayuso JM, Vitek R, Swick AD, Skala MC, Wisinski KB, Kimple RJ, Lambert PF, Beebe DJ. Effects of culture method on response to EGFR therapy in head and neck squamous cell carcinoma cells. Sci Rep. 2019 Aug 28;9(1):12480. doi: 10.1038/s41598-019-48764-3. PubMed PMID: 31462653; PubMed Central PMCID: PMC6713778.

Chen M, Choi S, Jung O, Wen T, Baum C, Thapa N, Lambert PF, Rapraeger AC, Anderson RA. The Specificity of EGF-Stimulated IQGAP1 Scaffold Towards the PI3K-Akt Pathway is Defined by the IQ3 motif. Sci Rep. 2019 Jun 24;9(1):9126. doi: 10.1038/s41598-019-45671-5. PubMed PMID: 31235839; PubMed Central PMCID: PMC6591252.

Eichelberg MR, Welch R, Guidry JT, Ali A, Ohashi M, Makielski KR, McChesney K, Van Sciver N, Lambert PF, Keles S, Kenney SC, Scott RS, Johannsen E. Epstein-Barr Virus Infection Promotes Epithelial Cell Growth by Attenuating Differentiation-Dependent Exit from the Cell Cycle. MBio. 2019 Aug 20;10(4). pii: e01332-19. doi: 10.1128/mBio.01332-19. PubMed PMID: 31431547; PubMed Central PMCID: PMC6703421.

He C, Lv X, Huang C, Angeletti PC, Hua G, Dong J, Zhou J, Wang Z, Ma B, Chen X, Lambert PF, Rueda BR, Davis JS, Wang C. A Human Papillomavirus-Independent Cervical Cancer Animal Model Reveals Unconventional Mechanisms of Cervical Carcinogenesis. Cell Rep. 2019 Mar 5;26(10):2636-2650.e5. doi: 10.1016/j.celrep.2019.02.004. PubMed PMID: 30840887.

Lambert PF, Sugden B. Viruses and Human Cancer. In: JE Niederhuber, JO Armitage, JH Doroshow, MB Kastan, JE Tepper (Eds.), Abeloff’s Clinical Oncology, Sixth Edition, pp. 165-179. Philadelphia: Elsevier Press, 2019.

Shin MK, Payne S, Bilger A, Matkowskyj KA, Carchman E, Meyer DS, Bentires-Alj M, Deming DA, Lambert PF. Activating Mutations in Pik3ca Contribute to Anal Carcinogenesis in the Presence or Absence of HPV-16 Oncogenes. Clin Cancer Res. 2019 Mar 15;25(6):1889-1900. doi: 10.1158/1078-0432.CCR-18-2843. Epub 2018 Dec 7. PubMed PMID: 30530704; PubMed Central PMCID: PMC6423984..

Spurgeon ME, Lambert PF. Sexual transmission of murine papillomavirus (MmuPV1) in Mus musculus. Elife. 2019 Oct 17;8. pii: e50056. doi: 10.7554/eLife.50056. PubMed PMID: 31621578.

Spurgeon ME, Uberoi A, McGregor SM, Wei T, Ward-Shaw E, Lambert PF. A Novel In Vivo Infection Model To Study Papillomavirus-Mediated Disease of the Female Reproductive Tract. MBio. 2019 Mar 5;10(2). pii: e00180-19. doi: 10.1128/mBio.00180-19. PubMed PMID: 30837335.

Wei T, Choi S, Buehler D, Anderson RA, Lambert PF. A PI3K/AKT Scaffolding Protein, IQ motif-containing GTPase Associating Protein 1 (IQGAP1), Promotes Head and Neck Carcinogenesis. Clin Cancer Res. 2019 Oct 9. pii: clincanres.1063.2019. doi: 10.1158/1078-0432.CCR-19-1063. [Epub ahead of print] PubMed PMID: 31597661.


Meyers JM, Grace M, Uberoi A, Lambert PF, Munger K. Inhibition of TGF-β and NOTCH Signaling by Cutaneous Papillomaviruses. Front Microbiol. 2018 Mar 8;9:389. doi: 10.3389/fmicb.2018.00389. eCollection 2018. Review. PubMed PMID: 29568286; PubMed Central PMCID: PMC5852067.

Munguía-Moreno JA, Díaz-Chavéz J, García-Villa E, Albino-Sanchez ME, Mendoza-Villanueva D, Ocadiz-Delgado R, Bonilla-Delgado J, Marín-Flores A, Cortés-Malagón EM, Alvarez-Rios E, Hidalgo-Miranda A, Üren A, Çelik H, Lambert PF, Gariglio P. Early synergistic interactions between the HPV16‑E7 oncoprotein and 17β-estradiol for repressing the expression of Granzyme B in a cervical cancer model. Int J Oncol. 2018 Aug;53(2):579-591. doi: 10.3892/ijo.2018.4432. Epub 2018 Jun 6. PubMed PMID: 29901186; PubMed Central PMCID: PMC6017153.

Nyman PE, Buehler D, Lambert PF. Loss of Function of Canonical Notch Signaling Drives Head and Neck Carcinogenesis. Clin Cancer Res. 2018 Dec 15;24(24):6308-6318. doi: 10.1158/1078-0432.CCR-17-3535. Epub 2018 Aug 7. PubMed PMID: 30087145; PubMed Central PMCID: PMC6295262.

Rodríguez-Uribe G, Serafín-Higuera N, Damian-Morales G, Cortés-Malagón EM, García-Hernández V, Verdejo-Torres O, Campos-Blázquez JP, Trejo-Muñoz CR, Contreras RG, Ocadiz-Delgado R, Palacios-Reyes C, Lambert PF, Griep AE, Mancilla-Percino T, Escobar-Herrera J, Álvarez-Ríos E, Ugarte-Briones C, Moreno J, Gariglio P, Bonilla-Delgado J. HPV16-E6 Oncoprotein Activates TGF-β and Wnt/β-Catenin Pathways in the Epithelium-Mesenchymal Transition of Cataracts in a Transgenic Mouse Model. Biomed Res Int. 2018 May 16;2018:2847873. doi: 10.1155/2018/2847873. eCollection 2018. PubMed PMID: 29888254; PubMed Central PMCID: PMC5977056.

Uberoi A, Yoshida S, Lambert PF. Development of an in vivo infection model to study Mouse papillomavirus-1 (MmuPV1). J Virol Methods. 2018 Mar;253:11-17. doi: 10.1016/j.jviromet.2017.12.002. Epub 2017 Dec 15. PubMed PMID: 29253496; PubMed Central PMCID: PMC5898621.

Zhao X, Brusadelli MG, Sauter S, Butsch Kovacic M, Zhang W, Romick-Rosendale LE, Lambert PF, Setchell KDR, Wells SI. Lipidomic Profiling Links the Fanconi Anemia Pathway to Glycosphingolipid Metabolism in Head and Neck Cancer Cells. Clin Cancer Res. 2018 Jun 1;24(11):2700-2709. doi: 10.1158/1078-0432.CCR-17-3686. PubMed PMID: 29530934; PubMed Central PMCID: PMC5984686.


Kraus RJ, Yu X, Cordes BA, Sathiamoorthi S, Iempridee T, Nawandar DM, Ma S, Romero-Masters JC, McChesney KG, Lin Z, Makielski KR, Lee DL, Lambert PF, Johannsen EC, Kenney SC, Mertz JE. Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter. PLoS Pathog. 2017 Jun 15;13(6):e1006404. doi: 10.1371/journal.ppat.1006404. eCollection 2017 Jun. PubMed PMID: 28617871; PubMed Central PMCID: PMC5487075.

Matrka MC, Watanabe M, Muraleedharan R, Lambert PF, Lane AN, Romick-Rosendale LE, Wells SI. Overexpression of the human DEK oncogene reprograms cellular metabolism and promotes glycolysis. PLoS One. 2017 May 30;12(5):e0177952. doi: 10.1371/journal.pone.0177952. eCollection 2017. PubMed PMID: 28558019; PubMed Central PMCID: PMC5448751

Meyers JM, Uberoi A, Grace M, Lambert PF, Munger K. Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-β Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation. PLoS Pathog. 2017 Jan 20;13(1):e1006171. doi: 10.1371/journal.ppat.1006171. PubMed PMID: 28107544; PubMed Central PMCID: PMC5287491.

Nawandar DM, Ohashi M, Djavadian R, Barlow E, Makielski K, Ali A, Lee D, Lambert PF, Johannsen E, Kenney SC. Differentiation-Dependent LMP1 Expression Is Required for Efficient Lytic Epstein-Barr Virus Reactivation in Epithelial Cells. J Virol. 2017 Mar 29;91(8). pii: e02438-16. doi: 10.1128/JVI.02438-16. Print 2017 Apr 15. PubMed PMID: 28179525; PubMed Central PMCID: PMC5375685.

Nowak K, Linzner D, Thrasher AJ, Lambert PF, Di WL, Burns SO. Absence of γ-Chain in Keratinocytes Alters Chemokine Secretion, Resulting in Reduced Immune Cell Recruitment. J Invest Dermatol. 2017 Oct;137(10):2120-2130. doi: 10.1016/j.jid.2017.05.024. Epub 2017 Jun 17. PubMed PMID: 28634034; PubMed Central PMCID: PMC5896775.

Spurgeon ME, Lambert PF. Human Papillomavirus and the Stroma: Bidirectional Crosstalk during the Virus Life Cycle and Carcinogenesis. Viruses. 2017 Aug 9;9(8). pii: E219. doi: 10.3390/v9080219. Review. PubMed PMID: 28792475; PubMed Central PMCID: PMC5580476.

Spurgeon ME, den Boon JA, Horswill M, Barthakur S, Forouzan O, Rader JS, Beebe DJ, Roopra A, Ahlquist P, Lambert PF. Human papillomavirus oncogenes reprogram the cervical cancer microenvironment independently of and synergistically with estrogen. Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):E9076-E9085. doi: 10.1073/pnas.1712018114. Epub 2017 Oct 9. PubMed PMID: 29073104; PubMed Central PMCID: PMC5664542.

Uberoi A, Lambert PF. Rodent Papillomaviruses. Viruses. 2017 Nov 27;9(12). pii: E362. doi: 10.3390/v9120362. Review. PubMed PMID: 29186900; PubMed Central PMCID: PMC5744137

Xue XY, Majerciak V, Uberoi A, Kim BH, Gotte D, Chen X, Cam M, Lambert PF, Zheng ZM. The full transcription map of mouse papillomavirus type 1 (MmuPV1) in mouse wart tissues. PLoS Pathog. 2017 Nov 27;13(11):e1006715. doi: 10.1371/journal.ppat.1006715. eCollection 2017 Nov. PubMed PMID: 29176795; PubMed Central PMCID: PMC5720830.

Yang YC, Liem A, Lambert PF, Sugden B. Dissecting the regulation of EBV's BART miRNAs in carcinomas. Virology. 2017 Mar 1;505:148-154. doi: 10.1016/j.virol.2017.02.013. [Epub ahead of print] PubMed PMID: 28259048; PubMed Central PMCID: PMC5504693.


Carchman EH, Matkowskyj KA, Meske L, Lambert PF. Dysregulation of Autophagy Contributes to Anal Carcinogenesis. PLoS One. 2016 Oct 5;11(10):e0164273. doi: 10.1371/journal.pone.0164273. PubMed PMID: 27706233; PubMed Central PMCID: PMC5051741.

Cicchini L, Westrich JA, Xu T, Vermeer DW, Berger JN, Clambey ET, Lee D, Song JI, Lambert PF, Greer RO, Lee JH, Pyeon D. Suppression of Antitumor Immune Responses by Human Papillomavirus through Epigenetic Downregulation of CXCL14. MBio. 2016 May 3;7(3). pii: e00270-16. doi: 10.1128/mBio.00270-16. PubMed PMID: 27143385; PubMed Central PMCID: PMC4959654.

Damian-Morales G, Serafín-Higuera N, Moreno-Eutimio MA, Cortés-Malagón EM, Bonilla-Delgado J, Rodríguez-Uribe G, Ocadiz-Delgado R, Lambert PF, Gariglio P. The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b(+)Gr1(+) Cells in a Transgenic Mouse Model. Biomed Res Int. 2016;2016:8091353. doi: 10.1155/2016/8091353. PubMed PMID: 27478837; PubMed Central PMCID: PMC4958469.

Gómez-Gómez Y, Organista-Nava J, Ocadiz-Delgado R, García-Villa E, Leyva-Vazquez MA, Illades-Aguiar B, Lambert PF, García-Carrancá A, Gariglio P. The expression of miR-21 and miR-143 is deregulated by the HPV16 E7 oncoprotein and 17β-estradiol. Int J Oncol. 2016 Aug;49(2):549-58. doi: 10.3892/ijo.2016.3575. Epub 2016 Jun 9. PubMed PMID: 27278606.

Jackson R, Rosa BA, Lameiras S, Cuninghame S, Bernard J, Floriano WB, Lambert PF, Nicolas A, Zehbe I. Functional variants of human papillomavirus type 16 demonstrate host genome integration and transcriptional alterations corresponding to their unique cancer epidemiology. BMC Genomics. 2016 Nov 2;17(1):851. PubMed PMID: 27806689; PubMed Central PMCID: PMC5094076. Erratum in:  BMC Genomics. 2016 Dec 12;17(1):1018. PubMed PMID: 27955615; PubMed Central PMCID: PMC5153815.

Lambert PF. Transgenic Mouse Models of Tumor Virus Action. Annu Rev Virol. 2016 Sep 29;3(1):473-489. PubMed PMID: 27741405.

Lee D, Norby K, Hayes M, Chiu YF, Sugden B, Lambert PF. Using Organotypic Epithelial Tissue Culture to Study the Human Papillomavirus Life Cycle. Curr Protoc Microbiol. 2016 May 6;41:14B.8.1-14B.8.19. doi: 10.1002/cpmc.4. PubMed PMID: 27153383; PubMed Central PMCID: PMC5035105.

Liu W, Yang R, Payne AS, Schowalter RM, Spurgeon ME, Lambert PF, Xu X, Buck CB, You J. Identifying the Target Cells and Mechanisms of Merkel Cell Polyomavirus Infection. Cell Host Microbe. 2016 Jun 8;19(6):775-87. doi: 10.1016/j.chom.2016.04.024. Epub 2016 May 19. PubMed PMID: 27212661; PubMed Central PMCID: PMC4900903.

Makielski KR, Lee D, Lorenz LD, Nawandar DM, Chiu YF, Kenney SC, Lambert PF. Human papillomavirus promotes Epstein-Barr virus maintenance and lytic reactivation in immortalized oral keratinocytes. Virology. 2016 Aug;495:52-62. doi: 10.1016/j.virol.2016.05.005. Epub 2016 May 11. PubMed PMID: 27179345; PubMed Central PMCID: PMC4912861.

Organista-Nava J, Gomez-Gomez Y, Ocadiz-Delgado R, Garcia-Villa E, Bonilla-Delgado J, Lagunas-Martinez A, Tapia JS, Lambert PF, Garcia-Carranca A, Gariglio P. The HPV16 E7 oncoprotein increases the expression of Oct3/4 and stemness-related genes and augments cell self-renewal. Virology. 2016 Sep 29;499:230-242. doi: 10.1016/j.virol.2016.09.020. PubMed PMID: 27693927.

Park S, Park JW, Pitot HC, Lambert PF. Loss of Dependence on Continued Expression of the Human Papillomavirus 16 E7 Oncogene in Cervical Cancers and Precancerous Lesions Arising in Fanconi Anemia Pathway-Deficient Mice. MBio. 2016 May 17;7(3). pii: e00628-16. doi: 10.1128/mBio.00628-16. PubMed PMID: 27190216; PubMed Central PMCID: PMC4895109.

Romick-Rosendale LE, Hoskins EE, Privette Vinnedge LM, Foglesong GD, Brusadelli MG, Potter SS, Komurov K, Brugmann SA, Lambert PF, Kimple RJ, Virts EL, Hanenberg H, Gillison ML, Wells SI. Defects in the Fanconi Anemia Pathway in Head and Neck Cancer Cells Stimulate Tumor Cell Invasion through DNA-PK and Rac1 Signaling. Clin Cancer Res. 2016 Apr 15;22(8):2062-73. doi: 10.1158/1078-0432.CCR-15-2209. Epub 2015 Nov 24. PubMed PMID: 26603260; PubMed Central PMCID: PMC4834270.

Smith MT, Guyton KZ, Gibbons CF, Fritz JM, Portier CJ, Rusyn I, DeMarini DM, Caldwell JC, Kavlock RJ, Lambert PF, Hecht SS, Bucher JR, Stewart BW, Baan RA, Cogliano VJ, Straif K. Key Characteristics of Carcinogens as a Basis for Organizing Data on Mechanisms of Carcinogenesis. Environ Health Perspect. 2016 Jun;124(6):713-21. doi: 10.1289/ehp.1509912. Epub 2015 Nov 24. PubMed PMID: 26600562; PubMed Central PMCID: PMC4892922.

Tan X, Lambert PF, Rapraeger AC, Anderson RA. Stress-Induced EGFR Trafficking: Mechanisms, Functions, and Therapeutic Implications. Trends Cell Biol. 2016 May;26(5):352-66. doi: 10.1016/j.tcb.2015.12.006. Epub 2016 Jan 27. Review. PubMed PMID: 26827089; PubMed Central PMCID: PMC5120732.

Thapa N, Tan X, Choi S, Lambert PF, Rapraeger AC, Anderson RA. The Hidden Conundrum of Phosphoinositide Signaling in Cancer. Trends Cancer. 2016 Jul;2(7):378-390. PubMed PMID: 27819060; PubMed Central PMCID: PMC5094282.

Uberoi A, Yoshida S, Frazer IH, Pitot HC, Lambert PF. Role of Ultraviolet Radiation in Papillomavirus-Induced Disease. PLoS Pathog. 2016 May 31;12(5):e1005664. doi: 10.1371/journal.ppat.1005664. eCollection 2016 May. PubMed PMID: 27244228; PubMed Central PMCID: PMC4887022.

Zhussupbekova S, Sinha R, Kuo P, Lambert PF, Frazer IH, Tuong ZK. A Mouse Model of Hyperproliferative Human Epithelium Validated by Keratin Profiling Shows an Aberrant Cytoskeletal Response to Injury. EBioMedicine. 2016 Jul;9:314-23. doi: 10.1016/j.ebiom.2016.06.011. Epub 2016 Jun 7. PubMed PMID: 27333029; PubMed Central PMCID: PMC4972546.


Adams AK, Hallenbeck GE, Casper KA, Patil YJ, Wilson KM, Kimple RJ, Lambert PF, Witte DP, Xiao W, Gillison ML, Wikenheiser-Brokamp KA, Wise-Draper TM, Wells SI. DEK promotes HPV-positive and -negative head and neck cancer cell proliferation. Oncogene. 2015 Feb 12;34(7):868-77. doi: 10.1038/onc.2014.15. Epub 2014 Mar 10. PubMed PMID: 24608431; PubMed Central PMCID: PMC4160430.

den Boon JA, Pyeon D, Wang SS, Horswill M, Schiffman M, Sherman M, Zuna RE, Wang Z, Hewitt SM, Pearson R, Schott M, Chung L, He Q, Lambert P, Walker J, Newton MA, Wentzensen N, Ahlquist P. Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling. Proc Natl Acad Sci U S A. 2015 Jun 23;112(25):E3255-64. doi: 10.1073/pnas.1509322112. Epub 2015 Jun 8. PubMed PMID: 26056290; PubMed Central PMCID: PMC4485108.

Gutiérrez J, García-Villa E, Ocadiz-Delgado R, Cortés-Malagón EM, Vázquez J, Roman-Rosales A, Alvarez-Rios E, Celik H, Romano MC, Üren A, Lambert PF, Gariglio P. Human papillomavirus type 16 E7 oncoprotein upregulates the retinoic acid receptor-beta expression in cervical cancer cell lines and K14E7 transgenic mice. Mol Cell Biochem. 2015 Oct;408(1-2):261-72. doi: 10.1007/s11010-015-2504-1. Epub 2015 Jul 15. PubMed PMID: 26173416; PubMed Central PMCID: Policy Exempt – non-U.S. gov’t. research support.

He C, Mao D, Hua G, Lv X, Chen X, Angeletti PC, Dong J, Remmenga SW, Rodabaugh KJ, Zhou J, Lambert PF, Yang P, Davis JS, Wang C. The Hippo/YAP pathway interacts with EGFR signaling and HPV oncoproteins to regulate cervical cancer progression. EMBO Mol Med. 2015 Sep 28;7(11):1426-49. doi: 10.15252/emmm.201404976. PubMed PMID: 26417066; PubMed Central PMCID: PMC4644376.

Kimble J, Bement WM, Chang Q, Cox BL, Drinkwater NR, Gourse RL, Hoskins AA, Huttenlocher A, Kreeger PK, Lambert PF, Mailick MR, Miyamoto S, Moss RL, O'Connor-Giles KM, Roopra A, Saha K, Seidel HS. Strategies from UW-Madison for rescuing biomedical research in the US. Elife. 2015 Jun 30;4:e09305. doi: 10.7554/eLife.09305. PubMed PMID: 26122792; PubMed Central PMCID: PMC4484056.

Matrka MC, Hennigan RF, Kappes F, DeLay ML, Lambert PF, Aronow BJ, Wells SI. DEK over-expression promotes mitotic defects and micronucleus formation. Cell Cycle. 2015;14(24):3939-53. doi: 10.1080/15384101.2015.1044177. Epub 2015 May 6. PubMed PMID: 25945971; PubMed Central PMCID: PMC4825741.

Nawandar DM, Wang A, Makielski K, Lee D, Ma S, Barlow E, Reusch J, Jiang R, Wille CK, Greenspan D, Greenspan JS, Mertz JE, Hutt-Fletcher L, Johannsen EC, Lambert PF, Kenney SC. Differentiation-Dependent KLF4 Expression Promotes Lytic Epstein-Barr Virus Infection in Epithelial Cells. PLoS Pathog. 2015 Oct 2;11(10):e1005195. doi: 10.1371/journal.ppat.1005195. eCollection 2015 Oct. PubMed PMID: 26431332; PubMed Central PMCID: PMC4592227.

Popoca-Cuaya M, Diaz-Chavez J, Hernandez-Monge J, Alvarez-Rios E, Lambert PF, Gariglio P. The HPV16 E6 oncoprotein and UVB irradiation inhibit the tumor suppressor TGFβ pathway in the epidermis of the K14E6 transgenic mouse. Exp Dermatol. 2015 Jun;24(6):430-5. doi: 10.1111/exd.12689. Epub 2015 Apr 16. PubMed PMID: 25776923; PubMed Central PMCID: Policy Exempt – non-U.S. gov’t. research support.

Spurgeon ME, Cheng J, Bronson RT, Lambert PF, DeCaprio JA. Tumorigenic activity of merkel cell polyomavirus T antigens expressed in the stratified epithelium of mice. Cancer Res. 2015 Mar 15;75(6):1068-79. doi: 10.1158/0008-5472.CAN-14-2425. Epub 2015 Jan 16. PubMed PMID: 25596282; PubMed Central PMCID: PMC4359959.

Stein AP, Saha S, Kraninger JL, Swick AD, Yu M, Lambert PF, Kimple RJ. Prevalence of Human Papillomavirus in Oropharyngeal Cancer: A Systematic Review.  Cancer J. 2015 May-Jun;21(3):138-46. doi: 10.1097/PPO.0000000000000115. PubMed PMID: 26049691; PubMed Central PMCID: PMC4459520.

Stein AP, Swick AD, Smith MA, Blitzer GC, Yang RZ, Saha S, Harari PM, Lambert PF, Liu CZ, Kimple RJ. Xenograft assessment of predictive biomarkers for standard head and neck cancer therapies. Cancer Med. 2015 May;4(5):699-712. doi: 10.1002/cam4.387. Epub 2015 Jan 26. PubMed PMID: 25619980; PubMed Central PMCID: PMC4430263.

Warren CJ, Xu T, Guo K, Griffin LM, Westrich JA, Lee D, Lambert PF, Santiago ML, Pyeon D. APOBEC3A functions as a restriction factor of human papillomavirus. J Virol. 2015 Jan;89(1):688-702. doi: 10.1128/JVI.02383-14. Epub 2014 Oct 29. PubMed PMID: 25355878; PubMed Central PMCID: PMC4301161.

Wille CK, Nawandar DM, Henning AN, Ma S, Oetting KM, Lee D, Lambert P, Johannsen EC, Kenney SC. 5-hydroxymethylation of the EBV genome regulates the latent to lytic switch. Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):E7257-65. doi: 10.1073/pnas.1513432112. Epub 2015 Dec 9. PubMed PMID: 26663912; PubMed Central PMCID: PMC4703011.


Jackson, R., Togtema, M., Lambert, P. F., and Zehbe, I.  Tumourigenesis Driven by the Human Papillomavirus Type 16 Asian-American E6 Variant in a Three-Dimensional Keratinocyte Model.  PLos One, 9(7): e101540, 2014.

Park, J. W., Nickel, K. P., Torres, A. D., Lee, D., Lambert, P. F., and Kimple, R. J.  Human Papillomavirus Type 16 E7 Oncoprotein Causes a Delay in Repair of DNA Damage.  Radiother. Oncol., 113(3): 337-344, 2014.

Park, J. W., Shin, M.-K., and Lambert, P. F.  High Incidence of Female Reproductive Tract Cancers in FA-Deficient HPV16-Transgenic Mice Correlates with E7’s Induction of DNA Damage Response, an Activity Mediated by E7’s Inactivation of Pocket Proteins.  Oncogene, 33(26): 3383-3391, 2014.

Son, J., Park, J. W., Lambert, P. F., and Chung, S.-H.  Requirement of Estrogen Receptor Alpha DNA-Binding Domain for HPV Oncogene-Induced Cervical Carcinogenesis in Mice.  Carcinogenesis, 35(2):  489-496, 2014.

Spurgeon, M. E., Chung, S.-H., and Lambert, P. F.  Recurrence of Cervical Cancer in Mice After Selective Estrogen Receptor Modulator Therapy.  Am. J. Pathol., 184(2):  530-540, 2014.

Stein, A. P., Saha, S., Liu, C. Z., Hartig, G. K., Lambert, P. F., and Kimple, R. J.  Influence of Handling Conditions on the Establishment and Propagation of Head and Neck Cancer Patient Derived Xenografts.  PLoS One, 9(6): e100995, 2014.

Stein, A. P., Saha, S., Yu, M., Kimple, R. J., and Lambert, P. F.  Prevalence of Human Papillomavirus in Oropharyngeal Squamous Cell Carcinoma in the United States Across Time.  Chem. Res. Toxicol., 27(4):  462-469, 2014.

Vieira, V. C., Leonard, B., White, E. A., Starrett, G. J., Temiz, N. A., Lorenz, L. D., Lee, D., Soares, M. A., Lambert, P. F., Howley, P. M., and Harris, R. S.  Human Papillomavirus E6 Triggers Upregulation of the Antiviral and Cancer Genomic DNA Deaminase APOBEC3B.  mBio, 5(6):  e02234-14, 2014.

Warren, C. J., Xu, T., Guo, K., Griffin, L. M., Westrich, J. A., Lee, D., Lambert, P. F., Santiago, M. L., and Pyeon, D.  APOBEC3A Functions As a Restriction Factor of Human Papillomavirus.  J. Virol., 89(1): 688-702, 2014.


Choyce, A., Yong, M., Narayan, S., Mattarollo, S. R., Liem, A., Lambert, P. F., Frazer, I. H., and Leggatt, G. R.  Expression of a Single, Viral Oncoprotein in Skin Epithelium Is Sufficient to Recruit Lymphocytes.  PLoS One, 8(2):e57798, 2013.

Chung, S.-H., Shin, M. K., Korach, K. S., and Lambert, P. F.  Requirement for Stromal Estrogen Receptor Alpha in Cervical Neoplasia.  Horm. Cancer, 4(1): 50-59, 2013.

Cortés-Malagón, E. M., Bonilla-Delgado, J., Díaz-Chávez, J., Hidalgo-Miranda, A., Romero-Cordoba, S., Üren, A., Çelik, H., McCormick, M., Munguía-Moreno, J. A., Ibarra-Sierra, E., Escobar-Herrera, J., Lambert, P. F., Mendoza-Villanueva, D., Bermudez-Cruz, R. M., and Gariglio, P.  Gene Expression Profile Regulated by the HPV16 E7 Oncoprotein and Estradiol in Cervical Tissue.  Virology, 447(1-2):  155-165, 2013.

Hernández-Monge, J., Garay, E., Raya-Sandino, A., Vargas-Sierra, O., Díaz-Chávez, J., Popoca-Cuaya, M., Lambert, P. F., González-Mariscal, L., and Gariglio, P.  Papillomavirus E6 Oncoprotein Up-Regulates Occludin and Z0-2 Expression in Ovariectomized Mice Epidermis.  Exp. Cell Res., 319(17):  2588-2603, 2013.

Johannsen, E., and Lambert, P. F.  Epigenetics of Human Papillomaviruses.  Virology, 445(1-2): 205-212, 2013.

Kimple, R. J., Harari, P. M., Torres, A. D., Yang, R. Z., Soriano, B. J., Yu, M., Armstrong, E. A., Blitzer, G. C., Smith, M. A., Lorenz, L. D., Lee, D., Yang, D. T., McCulloch, T. M., Hartig, G. K., and Lambert, P. F.  Development and Characterization of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinoma Tumorgrafts.  Clin. Cancer Res., 19(4): 855-864, 2013.

Kimple, R. J., Smith, M. A., Blitzer, G. C., Torres, A. D., Martin, J. A., Yang, R. Z., Peet, C. R., Lorenz, L. D., Nickel, K. P., Klingelhutz, A. J., Lambert, P. F., and Harari, P. M.  Enhanced Radiation Sensitivity in HPV-Positive Head and Neck Cancer.  Cancer Res., 73(15): 4791-4800, 2013.

Lambert, P. F., McBride, A., and Ulrich Bernard, H.  The Papillomavirus Episteme.  Virology (Special Issue), 445(1-2): 1, 2013.

Lorenz, L. D., Rivera Cardona, J., and Lambert, P. F.  Inactivation of p53 Rescues the Maintenance of High Risk HPV DNA Genomes Deficient in Expression of E6.  PLoS Pathog., 9(10): e1003717, 2013.

McNairn, A. J., Doucet, Y., Demaude, J., Brusadelli, M., Gordon, C. B., Uribe-Rivera, A., Lambert, P. F., Bouez, C., Breton, L., and Guasch, G.  TGFβ Signaling Regulates Lipogenesis in Human Sebaceous Glands Cells.  BMC Dermatol., 13(1): 2, 2013.

Michael, S., Lambert, P. F., and Strati, K.  The HPV16 Oncogenes Cause Aberrant Stem Cell Mobilization.  Virology, 443(2): 218-225, 2013.

Park, J. W., Shin, M.-K., Pitot, H. C., and Lambert, P. F.  High Incidence of HPV-Associated Head and Neck Cancers in FA Deficient Mice Is Associated with E7’s Induction of DNA Damage through Its Inactivation of Pocket Proteins.  PLoS One, 8(9):e75056, 2013.

Rivera, C., Simonson, S. J., S., Yamben, I. F., Shatadal, S., Nguyen, M. M., Beurg, M., Lambert, P. F., and Griep, A. E.  Requirement for Dlgh-1 in Planar Cell Polarity and Skeletogenesis during Vertebrate Development.  PLoS One, 8(1):e54410, 2013.

Spurgeon, M. E., and Lambert, P. F.  Merkel Cell Polyomavirus:  A Newly Discovered Human Virus with Oncogenic Potential.  Virology, 435(1):  118-130, 2013.


Bonilla-Delgado, J., Bulut, G., Liu, X., Cortés-Malagón, E. M., Schlegel, R., Flores-Maldonado, C., Contreras, R. G., Chung, S.-H., Lambert, P. F., Üren, A., and Gariglio, P.  The E6 Oncoprotein from HPV16 Enhances the Canonical Wnt/β-Catenin Pathway in Skin Epidermis in Vivo.  Mol. Cancer Res., 10: 250-258, 2012.

Hoskins, E. E., Morreale, R. J., Werner, S. P., Higginbotham, J. M., Laimins, L. A., Lambert, P. F., Brown, D. R., Gillison, M. L., Nuovo, G. J., Witte, D. P., Kim, M.-O., Davies, S. M., Mehta, P. A., Kovacic, M. B., Wikenheiser-Brokamp, K. A., and Wells, S. I.  The Fanconi Anemia Pathway Limits Human Papillomavirus Replication.  J. Virol., 86: 8131-8138, 2012.

Huang, H.-S., and Lambert, P. F.  Use of an in Vivo Animal Model for Assessing the Role of Integrin α6β4 and Syndecan-1 in Early Steps in Papillomavirus Infection.  Virology, 433: 395-400, 2012.

Huang, H.-S., Pyeon, D., Pearce, S. M., Lank, S. M., Griffin, L. M., Ahlquist, P., and Lambert, P. F.  Novel Antivirals Inhibit Early Steps in HPV Infection.  Antiviral Res., 93:  280-287, 2012.

Ibarra Sierra, E., Díaz Chávez, J., Cortés-Malagón, E. M., Uribe-Figueroa, L., Hidalgo-Miranda, A., Lambert, P. F., and Gariglio, P.  Differential Gene Expression between Skin and Cervix Induced by the E7 Oncoprotein in a Transgenic Mouse Model.  Virology, 433: 337-345, 2012.

Jabbar, S. F., Park, S., Schweizer, J., Berard-Bergery, M., Pitot, H. C., Lee, D., and Lambert, P. F.  Cervical Cancers Require the Continuous Expression of the Human Papillomavirus Type 16 E7 Oncoprotein Even in the Presence of the Viral E6 Oncoprotein.  Cancer Res., 72: 4008-4016, 2012.

Shin, M.-K., Pitot, H. C., and Lambert, P. F.  Pocket Proteins Suppress Head and Neck Cancer.  Cancer Res., 72: 1280-1289, 2012.

Shin, M.-K., Sage, J., and Lambert, P. F.  Inactivating All Three Rb Family Pocket Proteins Is Insufficient to Initiate Cervical Cancer.  Cancer Res., 72: 5418-5427, 2012. 

Togtema, M., Pichardo, S., Jackson, R., Lambert, P. F., Curiel, L., and Zehbe, I.  Sonoporation Delivery of Monoclonal Antibodies against Human Papillomavirus 16 E6 Restores p53 Expression in Transformed Cervical Keratinocytes.  PLoS One, 7(11):e50730, 2012.

Wen, Z., Pyeon, D., Wang, Y., Lambert, P., Xu, W., and Ahlquist, P.  Orphan Nuclear Receptor PNR/NR2E3 Stimulates p53 Functions by Enhancing p53 Acetylation.  Mol. Cell. Biol., 32:  26-35, 2012.

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National Committees: 

Member, National Institutes of Health Virology A Study Section, 2013-2019

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