UW madison
UW School of Medicine and Public Health
Carbone Cancer Center

Waclaw Szybalski, D.Sc.

Professor Emeritus of Oncology
Emeritus Program Member - UW Carbone Cancer Center

Ch.Eng., 1944, Chemistry, Institute of Technology, Lwów, Poland
M.Ch.Eng., 1945, Chemical Engineering, Institute of Technology, Gliwice, Poland
D.Sc., 1949, Microbial Biochemistry, Institute of Technology, Gdansk, Poland

Office: 
6th Fl (618A) McArdle Lab Bldg
Telephone: 
Office - (608) 238-3015, (608) 262-1259; Lab - (608) 262-2047
Email: 
szybalski@oncology.wisc.edu
Research Description: 

The objectives of our research on various aspects of genomics, including gene cloning, architecture and expression have been:
 (1) developing new methods for (i) ordering and in situ amplification of 30- to 100-kb fragments of large eukaryotic genomes, without conventional cloning, and (ii) ordered and automated sequencing of such large fragments by employing our SPEL-6 technique, based on primer assembly by hexamer ligation and primer walking;
(2) developing improved derivatives of pBAC vectors allowing conditional in vivo excision and amplification of 3- to 10-kb (cDNA) or 30- to 200 kb (genomic DNA) inserts in E. coli hosts [for more details, please see this abstract];
(3) the design and construction of novel regulatory circuits;
(4) the cloning of viral genes with dominant-lethal mutations as the basis for developing a new kind of immunity control against viral infection of plants and other organisms; and
(5) modifying and increasing specificity of restriction enzymes.

Selected Recent Publications: 

Szybalski, W.  The 50th Anniversary of Gene Therapy:  Beginnings and Present Realities.  Gene, 525(2): 151-154, 2013. 

Lobocka, M., and Szybalski, W. T.  Bacteriophages, Part A. Preface.  Adv. Virus Res., 82:  xiii-xv, 2012.

Lobocka, M., and Szybalski, W. T.  Phages.  Preface.  Adv. Virus Res., 83:  xvii-xviii, 2012.

Malecki, M., and Szybalski, W.  Isolation of Single, Intact Chromosomes from Single, Selected Ovarian Cancer Cells for in Situ Hybridization and Sequencing.  Gene, 493: 132-139, 2012.

Perform a PubMed search for additional Szybalski publications

A complete list of Publications is available.

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