]r:@kn3Ւ=,FN\ I VkLL<\ZޔO(Gkokyvxb2럾zՕk]^n{}y*δwjbIQbxD./.u1iל#v<:ڼR^-sBƭǗ߯^X2ͥ%cYZ, wAUx}`#Jֱ1&@'AX1gCBCo9@zn'ptmGsz_^}Ryy{gG`ANoz<^X;KV:PFͧ˫'G/Os<;ws4FGQ;uH>8>|յn?lTH|~fϩypF_GߨCZ߷4"567?ḍ{ٻ$W>O;c>X%;k>il݁P`熨4vn :Y[wN   Jأ >T>=efS#Yv@,2 vПkjd#N lb ,[topJ Tzt:?7 <=MLV ]SAyxd83y$Qj((d722<`ͬT~뻶l#q]׶<ꆁ~W{z;}6jo'C{L+~}h3uѬtzvy[)޼jWyc{f}X5`\zS8GKIP<i@GɃJD\xp<ݿzuW[g2s.GU+r4x8=zܫ?`' 6+*LG|0=^~Quc`!o'" B[&#*!;IVYVPya7!\ԍr٦"M%z&L|(Pf'O1۷پ6b."\`uG 24Y KvsWSm@ L+rHo!F_ q7VfPonx,͘G'f `9P#ʔI2#E" o@Uy*rs8; yCqr+=>%gi" |G;]uCS`=N?/5p2aHgFH/O">3"K?d+<C (ڒlm}98S WhK Pmt\HKSzv]{rw(؎"ї.J ]&+k:S+v* --'+"P}*ay14΀v!/!e]_xl |d@)O1q=rڍD-? kͯAvj5*t&J{0kdynpUO]2wJpf-05!uhx>ye,\px]g`(\Х89=b~QO"]%3s=],QHeéf:}V|eQT^Tg "Nφ+ѻ 5 A3GuMHSBDf/-ϓC 0Z׭2X1&+JNDs{bq9KyFoaCscm']1ttޣ\cnkkӹw8#2%1h>ԙ?O؝_:%Zm^7p>7%k2 sg谹c 6Dqas =8t3>w9wB9 =_A{So>ߎ>y%1`8k/:R&t2Z-L(rJ>xBb&-=>VkCK*nK07mEy/'8~79݀*<]jWo[0Wua/zݣN LZ5(_7`vϪn¿Uk7k{OvEhmKwlHb&2v q7hiķ[B؉RIӜU" C]`Eay+V7yVh1H϶Sm~P'B! VK!o L'0)7rκ:яF@܃3ŝԒǸ4e2Fd{6Ή<9ΐ@Ǐ2(&4&Ȉ.q cSpI'֦eHWv0^o w3M+kg ;%Ǎ]K_ܵ(n9ӆm(v_]`D$$"8>XC oB,dV+E=JQJOB8b7m|fM%V4hۊij]ooI'i_mU'v*EC Ug3ar:N|]|0)oVߵt}qGyt<$^ 0$1+T0y" M vqmȺX(7_ųZ@BF%S,PJgTx=/c *[\䑷Erc6,$(ޔE][ѐ*>䧌.=ge Vv k8g!mnrR,8L >K44%e!%nLRF'}\+M* rZ0/fw5ݝmxUm%-B^XcNU_7^m4_鉬?ZZi]kj͗],Acpb[R R#6@͇u C=?,ϋ`#X%EXy}ͦV"LX o;K"/E (jApPknŔ)ͤ ]e_0KfAuo(>Xi)} 4IDyx9|G똯d|(h-.`aS@y2_z? D'2'Jx屝3{.LhyATGWr2%+[兺V@Bw2Sl*}$sI+6ZƺK{+ A " se]%CZ]>,C74,@A6>K Wx"iЋ2z‰\-}%zh`L&`? ]$/ES.!Lj Р2: \d/׃Xc%t(3D\K@waJfΪPGaNZF̸໤,2˲]IÍsA"Lr\kq,i\:(Mz@br yq^bx zWq p)_Qgش=;$HYŹYCb3rl\&O9ws*/w!xpmƲ"#Y!qZE`!qW- ~+\du17gBʡʾaps@8@Nqq2Y?#q2wF,cDaW6:o^̨2;ˎϹlnÓy)k ce9ceV6υՀte\JF~~3$qSFyُ>X%lk`aYLNԣǜX{ZxVB}Pϋiȹ^AZG\S|ض=`x\x0FSaiW7yޕȶx*~ͬ{k}z<<7~`O1}?Smxbb9&0 K ǔL 4 {5zĩszO~E&i@[ zuvIFu3հLEY&EASߛ.HVܨ]T2b#1'ɉf}5Fv}JV!4to-́]{N~-Mt|V,+M/Rl䯛&2 E0{b?] زaXM3l.v1jN|n _X{b?fVLi]ΝM^G zh,N-%,2+|fӮHI[lIþQ+Oaʍ}ط߼ާoƾy')o "Ic_ύ}N7 Src_ i쫹 W}~J,ҹJNw Sk+wk[zNymNymXSHc;)mk is絕;嵭bM!}rU)ϝV׶6}rUis絕;嵭"]¾k9坰/4)mymVn2̍vצoNymk7rcO4}'Nu#XRk๘Z{YFs?,݆'6ؕvAulA~ʹO8Ӏ,3Gp=b c4s6A)'}zލ;s2i%'7*0Kt%UyAj `*"/`'6Te_Fԗ Kd5P^T**-zo=,vhg=R[)1uqaKWx&hf!0K A;"3 QS]JAFM^!9HDbI(גP&o!?@ ьa?4 Dpx3X(H ^`ynP3 Q,hÀ r2Bݡ_-2#bPkV$:DyQ(G(So g _SˬMċCͫ*?*ۥd88fWe=ss77:?cR )؞ ZԕrB^Z f}C:[+ܞCyd{S%,R_YocLY}in@/Tr)Txg{BR*zEW=(ZKOA}NH;"Q ܳ]dT;!lPڎhux52ߧEt[?:Q|Nt,6N~|Ɛg'tLHbU& >.+dxM![%RAhxR9pK|=I~-{T2L&Sy,Z3sHݲE1lmҷa5J`{T،q'WrYbzP:|Ou(EFRLr- 59O_d删j:.5NiyG$N1V'1iPƒpLzB[(M% %CRvyWE$ ^ۢWg+z0b%hD#hC2^=k0B|K}6Z|/24 chϲD_ X-- ^#8AS*u"#\^ F7MnS-Uȃ)&C3Gʆܚhh.w\"_B]HQox&ȕrJ\L]eT~0 Ŏaѻd Y AH`|ADdH 4`K–8Eϧ~W_FE+O;>qm[OȾM7ִk`!Ubg.7*/u}۽ty$ʊUNA֖gzjEy$e%Ǎx導w,d1W"愩0T&"r *AlV6-;<)D7޽ix;'_[-̺4ՂBEhZ"|K .t#[ܲ O]y0ΐp)X$XNVu2Mq;9@IǠ.+ w;xݖG ʼnNc/&&/תuҨW=qMF_ǽJbL1IyA8cqГ'@88!Q qaޢ=` m?_@>zj;11l]wi\6 FB:%Vhǭ@ŎB)p8E< Ϭ-PðM<_IVbwu<Sʥt@f;Ce8IJ Z4B\YAFtXˍ݈'`g [r=ENL1u!/5m jIɟy `dP =$ &5ZF2[XDdͯ&/-:Qۡ<}drË99,bdXg$ɚx4:KZHU'+2]rko2~}'DY.;ꄛn6qӴ^ݩVjݨ}ӯTjOnZ~7udue$6.q/> fPmv2LLeg䙗lu$~ZSճje^٪T&|vωnNbMɷCYx'^BkTB=!WozIaך]k{a=pZ$}373x7]ÝCpBbTnsƜ\T1 nnUp;ګ(P.bJL 9hx|%d]''xdCrِn!D7hȆbĒE䒊\6,faK˒ՋE7Di;/1x׾?g ]r:@kn3Ւ=,FN\ I VkLL<\ZޔO(Gkokyvxb2럾zՕk]^n{}y*δwjbIQbxD./.u1iל#v<:ڼR^-sBƭǗ߯^X2ͥ%cYZ, wAUx}`#Jֱ1&@'AX1gCBCo9@zn'ptmGsz_^}Ryy{gG`ANoz<^X;KV:PFͧ˫'G/Os<;ws4FGQ;uH>8>|յn?lTH|~fϩypF_GߨCZ߷4"567?ḍ{ٻ$W>O;c>X%;k>il݁P`熨4vn :Y[wN   Jأ >T>=efS#Yv@,2 vПkjd#N lb ,[topJ Tzt:?7 <=MLV ]SAyxd83y$Qj((d722<`ͬT~뻶l#q]׶<ꆁ~W{z;}6jo'C{L+~}h3uѬtzvy[)޼jWyc{f}X5`\zS8GKIP<i@GɃJD\xp<ݿzuW[g2s.GU+r4x8=zܫ?`' 6+*LG|0=^~Quc`!o'" B[&#*!;IVYVPya7!\ԍr٦"M%z&L|(Pf'O1۷پ6b."\`uG 24Y KvsWSm@ L+rHo!F_ q7VfPonx,͘G'f `9P#ʔI2#E" o@Uy*rs8; yCqr+=>%gi" |G;]uCS`=N?/5p2aHgFH/O">3"K?d+<C (ڒlm}98S WhK Pmt\HKSzv]{rw(؎"ї.J ]&+k:S+v* --'+"P}*ay14΀v!/!e]_xl |d@)O1q=rڍD-? kͯAvj5*t&J{0kdynpUO]2wJpf-05!uhx>ye,\px]g`(\Х89=b~QO"]%3s=],QHeéf:}V|eQT^Tg "Nφ+ѻ 5 A3GuMHSBDf/-ϓC 0Z׭2X1&+JNDs{bq9KyFoaCscm']1ttޣ\cnkkӹw8#2%1h>ԙ?O؝_:%Zm^7p>7%k2 sg谹c 6Dqas =8t3>w9wB9 =_A{So>ߎ>y%1`8k/:R&t2Z-L(rJ>xBb&-=>VkCK*nK07mEy/'8~79݀*<]jWo[0Wua/zݣN LZ5(_7`vϪn¿Uk7k{OvEhmKwlHb&2v q7hiķ[B؉RIӜU" C]`Eay+V7yVh1H϶Sm~P'B! VK!o L'0)7rκ:яF@܃3ŝԒǸ4e2Fd{6Ή<9ΐ@Ǐ2(&4&Ȉ.q cSpI'֦eHWv0^o w3M+kg ;%Ǎ]K_ܵ(n9ӆm(v_]`D$$"8>XC oB,dV+E=JQJOB8b7m|fM%V4hۊij]ooI'i_mU'v*EC Ug3ar:N|]|0)oVߵt}qGyt<$^ 0$1+T0y" M vqmȺX(7_ųZ@BF%S,PJgTx=/c *[\䑷Erc6,$(ޔE][ѐ*>䧌.=ge Vv k8g!mnrR,8L >K44%e!%nLRF'}\+M* rZ0/fw5ݝmxUm%-B^XcNU_7^m4_鉬?ZZi]kj͗],Acpb[R R#6@͇u C=?,ϋ`#X%EXy}ͦV"LX o;K"/E (jApPknŔ)ͤ ]e_0KfAuo(>Xi)} 4IDyx9|G똯d|(h-.`aS@y2_z? D'2'Jx屝3{.LhyATGWr2%+[兺V@Bw2Sl*}$sI+6ZƺK{+ A " se]%CZ]>,C74,@A6>K Wx"iЋ2z‰\-}%zh`L&`? ]$/ES.!Lj Р2: \d/׃Xc%t(3D\K@waJfΪPGaNZF̸໤,2˲]IÍsA"Lr\kq,i\:(Mz@br yq^bx zWq p)_Qgش=;$HYŹYCb3rl\&O9ws*/w!xpmƲ"#Y!qZE`!qW- ~+\du17gBʡʾaps@8@Nqq2Y?#q2wF,cDaW6:o^̨2;ˎϹlnÓy)k ce9ceV6υՀte\JF~~3$qSFyُ>X%lk`aYLNԣǜX{ZxVB}Pϋiȹ^AZG\S|ض=`x\x0FSaiW7yޕȶx*~ͬ{k}z<<7~`O1}?Smxbb9&0 K ǔL 4 {5zĩszO~E&i@[ zuvIFu3հLEY&EASߛ.HVܨ]T2b#1'ɉf}5Fv}JV!4to-́]{N~-Mt|V,+M/Rl䯛&2 E0{b?] زaXM3l.v1jN|n _X{b?fVLi]ΝM^G zh,N-%,2+|fӮHI[lIþQ+Oaʍ}ط߼ާoƾy')o "Ic_ύ}N7 Src_ i쫹 W}~J,ҹJNw Sk+wk[zNymNymXSHc;)mk is絕;嵭bM!}rU)ϝV׶6}rUis絕;嵭"]¾k9坰/4)mymVn2̍vצoNymk7rcO4}'Nu#XRk๘Z{YFs?,݆'6ؕvAulA~ʹO8Ӏ,3Gp=b c4s6A)'}zލ;s2i%'7*0Kt%UyAj `*"/`'6Te_Fԗ Kd5P^T**-zo=,vhg=R[)1uqaKWx&hf!0K A;"3 QS]JAFM^!9HDbI(גP&o!?@ ьa?4 Dpx3X(H ^`ynP3 Q,hÀ r2Bݡ_-2#bPkV$:DyQ(G(So g _SˬMċCͫ*?*ۥd88fWe=ss77:?cR )؞ ZԕrB^Z f}C:[+ܞCyd{S%,R_YocLY}in@/Tr)Txg{BR*zEW=(ZKOA}NH;"Q ܳ]dT;!lPڎhux52ߧEt[?:Q|Nt,6N~|Ɛg'tLHbU& >.+dxM![%RAhxR9pK|=I~-{T2L&Sy,Z3sHݲE1lmҷa5J`{T،q'WrYbzP:|Ou(EFRLr- 59O_d删j:.5NiyG$N1V'1iPƒpLzB[(M% %CRvyWE$ ^ۢWg+z0b%hD#hC2^=k0B|K}6Z|/24 chϲD_ X-- ^#8AS*u"#\^ F7MnS-Uȃ)&C3Gʆܚhh.w\"_B]HQox&ȕrJ\L]eT~0 Ŏaѻd Y AH`|ADdH 4`K–8Eϧ~W_FE+O;>qm[OȾM7ִk`!Ubg.7*/u}۽ty$ʊUNA֖gzjEy$e%Ǎx導w,d1W"愩0T&"r *AlV6-;<)D7޽ix;'_[-̺4ՂBEhZ"|K .t#[ܲ O]y0ΐp)X$XNVu2Mq;9@IǠ.+ w;xݖG ʼnNc/&&/תuҨW=qMF_ǽJbL1IyA8cqГ'@88!Q qaޢ=` m?_@>zj;11l]wi\6 FB:%Vhǭ@ŎB)p8E< Ϭ-PðM<_IVbwu<Sʥt@f;Ce8IJ Z4B\YAFtXˍ݈'`g [r=ENL1u!/5m jIɟy `dP =$ &5ZF2[XDdͯ&/-:Qۡ<}drË99,bdXg$ɚx4:KZHU'+2]rko2~}'DY.;ꄛn6qӴ^ݩVjݨ}ӯTjOnZ~7udue$6.q/> fPmv2LLeg䙗lu$~ZSճje^٪T&|vωnNbMɷCYx'^BkTB=!WozIaך]k{a=pZ$}373x7]ÝCpBbTnsƜ\T1 nnUp;ګ(P.bJL 9hx|%d]''xdCrِn!D7hȆbĒE䒊\6,faK˒ՋE7Di;/1x׾?g

Download Bowtie indexes

mPrime uses bowtie to align primers to genomic or other sequences in order to identify potential priming/mispriming sites. Indexes for the mouse, human, and rat genomes were built from the DNA sequence (fasta) files in Build 71 of Ensembl. Three sets of indexes are available for each species. In order to perform the alignment on your own computer, download the appropriate archive file and unzip to any folder on your computer. To align primers to genomic sequence you should download the "by chromosome" set; it will allow the alignments to run much faster (and in less memory) than if you use the "whole genome" set. Indexes of cDNAs for the complete set of Ensembl 69 known and novel transcripts, as well as pseudogene predictions are also provided to facilitate alignment of primers to be used for RT-PCR.

Note: Do not attempt to use the "whole genome" indexes with 32-bit versions of mPrime. The 32-bit version of bowtie quickly runs out of memory when the whole genome indexes are used. Using the "by chromosome" indexes and the "Search all indexes in directory" option in the mispriming dialog works well even on fairly modest hardware.

Indexes by chromosome

cDNA indexes

Whole genome indexes

]r:@kn3Ւ=,FN\ I VkLL<\ZޔO(Gkokyvxb2럾zՕk]^n{}y*δwjbIQbxD./.u1iל#v<:ڼR^-sBƭǗ߯^X2ͥ%cYZ, wAUx}`#Jֱ1&@'AX1gCBCo9@zn'ptmGsz_^}Ryy{gG`ANoz<^X;KV:PFͧ˫'G/Os<;ws4FGQ;uH>8>|յn?lTH|~fϩypF_GߨCZ߷4"567?ḍ{ٻ$W>O;c>X%;k>il݁P`熨4vn :Y[wN   Jأ >T>=efS#Yv@,2 vПkjd#N lb ,[topJ Tzt:?7 <=MLV ]SAyxd83y$Qj((d722<`ͬT~뻶l#q]׶<ꆁ~W{z;}6jo'C{L+~}h3uѬtzvy[)޼jWyc{f}X5`\zS8GKIP<i@GɃJD\xp<ݿzuW[g2s.GU+r4x8=zܫ?`' 6+*LG|0=^~Quc`!o'" B[&#*!;IVYVPya7!\ԍr٦"M%z&L|(Pf'O1۷پ6b."\`uG 24Y KvsWSm@ L+rHo!F_ q7VfPonx,͘G'f `9P#ʔI2#E" o@Uy*rs8; yCqr+=>%gi" |G;]uCS`=N?/5p2aHgFH/O">3"K?d+<C (ڒlm}98S WhK Pmt\HKSzv]{rw(؎"ї.J ]&+k:S+v* --'+"P}*ay14΀v!/!e]_xl |d@)O1q=rڍD-? kͯAvj5*t&J{0kdynpUO]2wJpf-05!uhx>ye,\px]g`(\Х89=b~QO"]%3s=],QHeéf:}V|eQT^Tg "Nφ+ѻ 5 A3GuMHSBDf/-ϓC 0Z׭2X1&+JNDs{bq9KyFoaCscm']1ttޣ\cnkkӹw8#2%1h>ԙ?O؝_:%Zm^7p>7%k2 sg谹c 6Dqas =8t3>w9wB9 =_A{So>ߎ>y%1`8k/:R&t2Z-L(rJ>xBb&-=>VkCK*nK07mEy/'8~79݀*<]jWo[0Wua/zݣN LZ5(_7`vϪn¿Uk7k{OvEhmKwlHb&2v q7hiķ[B؉RIӜU" C]`Eay+V7yVh1H϶Sm~P'B! VK!o L'0)7rκ:яF@܃3ŝԒǸ4e2Fd{6Ή<9ΐ@Ǐ2(&4&Ȉ.q cSpI'֦eHWv0^o w3M+kg ;%Ǎ]K_ܵ(n9ӆm(v_]`D$$"8>XC oB,dV+E=JQJOB8b7m|fM%V4hۊij]ooI'i_mU'v*EC Ug3ar:N|]|0)oVߵt}qGyt<$^ 0$1+T0y" M vqmȺX(7_ųZ@BF%S,PJgTx=/c *[\䑷Erc6,$(ޔE][ѐ*>䧌.=ge Vv k8g!mnrR,8L >K44%e!%nLRF'}\+M* rZ0/fw5ݝmxUm%-B^XcNU_7^m4_鉬?ZZi]kj͗],Acpb[R R#6@͇u C=?,ϋ`#X%EXy}ͦV"LX o;K"/E (jApPknŔ)ͤ ]e_0KfAuo(>Xi)} 4IDyx9|G똯d|(h-.`aS@y2_z? D'2'Jx屝3{.LhyATGWr2%+[兺V@Bw2Sl*}$sI+6ZƺK{+ A " se]%CZ]>,C74,@A6>K Wx"iЋ2z‰\-}%zh`L&`? ]$/ES.!Lj Р2: \d/׃Xc%t(3D\K@waJfΪPGaNZF̸໤,2˲]IÍsA"Lr\kq,i\:(Mz@br yq^bx zWq p)_Qgش=;$HYŹYCb3rl\&O9ws*/w!xpmƲ"#Y!qZE`!qW- ~+\du17gBʡʾaps@8@Nqq2Y?#q2wF,cDaW6:o^̨2;ˎϹlnÓy)k ce9ceV6υՀte\JF~~3$qSFyُ>X%lk`aYLNԣǜX{ZxVB}Pϋiȹ^AZG\S|ض=`x\x0FSaiW7yޕȶx*~ͬ{k}z<<7~`O1}?Smxbb9&0 K ǔL 4 {5zĩszO~E&i@[ zuvIFu3հLEY&EASߛ.HVܨ]T2b#1'ɉf}5Fv}JV!4to-́]{N~-Mt|V,+M/Rl䯛&2 E0{b?] زaXM3l.v1jN|n _X{b?fVLi]ΝM^G zh,N-%,2+|fӮHI[lIþQ+Oaʍ}ط߼ާoƾy')o "Ic_ύ}N7 Src_ i쫹 W}~J,ҹJNw Sk+wk[zNymNymXSHc;)mk is絕;嵭bM!}rU)ϝV׶6}rUis絕;嵭"]¾k9坰/4)mymVn2̍vצoNymk7rcO4}'Nu#XRk๘Z{YFs?,݆'6ؕvAulA~ʹO8Ӏ,3Gp=b c4s6A)'}zލ;s2i%'7*0Kt%UyAj `*"/`'6Te_Fԗ Kd5P^T**-zo=,vhg=R[)1uqaKWx&hf!0K A;"3 QS]JAFM^!9HDbI(גP&o!?@ ьa?4 Dpx3X(H ^`ynP3 Q,hÀ r2Bݡ_-2#bPkV$:DyQ(G(So g _SˬMċCͫ*?*ۥd88fWe=ss77:?cR )؞ ZԕrB^Z f}C:[+ܞCyd{S%,R_YocLY}in@/Tr)Txg{BR*zEW=(ZKOA}NH;"Q ܳ]dT;!lPڎhux52ߧEt[?:Q|Nt,6N~|Ɛg'tLHbU& >.+dxM![%RAhxR9pK|=I~-{T2L&Sy,Z3sHݲE1lmҷa5J`{T،q'WrYbzP:|Ou(EFRLr- 59O_d删j:.5NiyG$N1V'1iPƒpLzB[(M% %CRvyWE$ ^ۢWg+z0b%hD#hC2^=k0B|K}6Z|/24 chϲD_ X-- ^#8AS*u"#\^ F7MnS-Uȃ)&C3Gʆܚhh.w\"_B]HQox&ȕrJ\L]eT~0 Ŏaѻd Y AH`|ADdH 4`K–8Eϧ~W_FE+O;>qm[OȾM7ִk`!Ubg.7*/u}۽ty$ʊUNA֖gzjEy$e%Ǎx導w,d1W"愩0T&"r *AlV6-;<)D7޽ix;'_[-̺4ՂBEhZ"|K .t#[ܲ O]y0ΐp)X$XNVu2Mq;9@IǠ.+ w;xݖG ʼnNc/&&/תuҨW=qMF_ǽJbL1IyA8cqГ'@88!Q qaޢ=` m?_@>zj;11l]wi\6 FB:%Vhǭ@ŎB)p8E< Ϭ-PðM<_IVbwu<Sʥt@f;Ce8IJ Z4B\YAFtXˍ݈'`g [r=ENL1u!/5m jIɟy `dP =$ &5ZF2[XDdͯ&/-:Qۡ<}drË99,bdXg$ɚx4:KZHU'+2]rko2~}'DY.;ꄛn6qӴ^ݩVjݨ}ӯTjOnZ~7udue$6.q/> fPmv2LLeg䙗lu$~ZSճje^٪T&|vωnNbMɷCYx'^BkTB=!WozIaך]k{a=pZ$}373x7]ÝCpBbTnsƜ\T1 nnUp;ګ(P.bJL 9hx|%d]''xdCrِn!D7hȆbĒE䒊\6,faK˒ՋE7Di;/1x׾?g Last modified on Tuesday, 25-Apr-2017 07:20:12 CDT.

]r:@kn3Ւ=,FN\ I VkLL<\ZޔO(Gkokyvxb2럾zՕk]^n{}y*δwjbIQbxD./.u1iל#v<:ڼR^-sBƭǗ߯^X2ͥ%cYZ, wAUx}`#Jֱ1&@'AX1gCBCo9@zn'ptmGsz_^}Ryy{gG`ANoz<^X;KV:PFͧ˫'G/Os<;ws4FGQ;uH>8>|յn?lTH|~fϩypF_GߨCZ߷4"567?ḍ{ٻ$W>O;c>X%;k>il݁P`熨4vn :Y[wN   Jأ >T>=efS#Yv@,2 vПkjd#N lb ,[topJ Tzt:?7 <=MLV ]SAyxd83y$Qj((d722<`ͬT~뻶l#q]׶<ꆁ~W{z;}6jo'C{L+~}h3uѬtzvy[)޼jWyc{f}X5`\zS8GKIP<i@GɃJD\xp<ݿzuW[g2s.GU+r4x8=zܫ?`' 6+*LG|0=^~Quc`!o'" B[&#*!;IVYVPya7!\ԍr٦"M%z&L|(Pf'O1۷پ6b."\`uG 24Y KvsWSm@ L+rHo!F_ q7VfPonx,͘G'f `9P#ʔI2#E" o@Uy*rs8; yCqr+=>%gi" |G;]uCS`=N?/5p2aHgFH/O">3"K?d+<C (ڒlm}98S WhK Pmt\HKSzv]{rw(؎"ї.J ]&+k:S+v* --'+"P}*ay14΀v!/!e]_xl |d@)O1q=rڍD-? kͯAvj5*t&J{0kdynpUO]2wJpf-05!uhx>ye,\px]g`(\Х89=b~QO"]%3s=],QHeéf:}V|eQT^Tg "Nφ+ѻ 5 A3GuMHSBDf/-ϓC 0Z׭2X1&+JNDs{bq9KyFoaCscm']1ttޣ\cnkkӹw8#2%1h>ԙ?O؝_:%Zm^7p>7%k2 sg谹c 6Dqas =8t3>w9wB9 =_A{So>ߎ>y%1`8k/:R&t2Z-L(rJ>xBb&-=>VkCK*nK07mEy/'8~79݀*<]jWo[0Wua/zݣN LZ5(_7`vϪn¿Uk7k{OvEhmKwlHb&2v q7hiķ[B؉RIӜU" C]`Eay+V7yVh1H϶Sm~P'B! VK!o L'0)7rκ:яF@܃3ŝԒǸ4e2Fd{6Ή<9ΐ@Ǐ2(&4&Ȉ.q cSpI'֦eHWv0^o w3M+kg ;%Ǎ]K_ܵ(n9ӆm(v_]`D$$"8>XC oB,dV+E=JQJOB8b7m|fM%V4hۊij]ooI'i_mU'v*EC Ug3ar:N|]|0)oVߵt}qGyt<$^ 0$1+T0y" M vqmȺX(7_ųZ@BF%S,PJgTx=/c *[\䑷Erc6,$(ޔE][ѐ*>䧌.=ge Vv k8g!mnrR,8L >K44%e!%nLRF'}\+M* rZ0/fw5ݝmxUm%-B^XcNU_7^m4_鉬?ZZi]kj͗],Acpb[R R#6@͇u C=?,ϋ`#X%EXy}ͦV"LX o;K"/E (jApPknŔ)ͤ ]e_0KfAuo(>Xi)} 4IDyx9|G똯d|(h-.`aS@y2_z? D'2'Jx屝3{.LhyATGWr2%+[兺V@Bw2Sl*}$sI+6ZƺK{+ A " se]%CZ]>,C74,@A6>K Wx"iЋ2z‰\-}%zh`L&`? ]$/ES.!Lj Р2: \d/׃Xc%t(3D\K@waJfΪPGaNZF̸໤,2˲]IÍsA"Lr\kq,i\:(Mz@br yq^bx zWq p)_Qgش=;$HYŹYCb3rl\&O9ws*/w!xpmƲ"#Y!qZE`!qW- ~+\du17gBʡʾaps@8@Nqq2Y?#q2wF,cDaW6:o^̨2;ˎϹlnÓy)k ce9ceV6υՀte\JF~~3$qSFyُ>X%lk`aYLNԣǜX{ZxVB}Pϋiȹ^AZG\S|ض=`x\x0FSaiW7yޕȶx*~ͬ{k}z<<7~`O1}?Smxbb9&0 K ǔL 4 {5zĩszO~E&i@[ zuvIFu3հLEY&EASߛ.HVܨ]T2b#1'ɉf}5Fv}JV!4to-́]{N~-Mt|V,+M/Rl䯛&2 E0{b?] زaXM3l.v1jN|n _X{b?fVLi]ΝM^G zh,N-%,2+|fӮHI[lIþQ+Oaʍ}ط߼ާoƾy')o "Ic_ύ}N7 Src_ i쫹 W}~J,ҹJNw Sk+wk[zNymNymXSHc;)mk is絕;嵭bM!}rU)ϝV׶6}rUis絕;嵭"]¾k9坰/4)mymVn2̍vצoNymk7rcO4}'Nu#XRk๘Z{YFs?,݆'6ؕvAulA~ʹO8Ӏ,3Gp=b c4s6A)'}zލ;s2i%'7*0Kt%UyAj `*"/`'6Te_Fԗ Kd5P^T**-zo=,vhg=R[)1uqaKWx&hf!0K A;"3 QS]JAFM^!9HDbI(גP&o!?@ ьa?4 Dpx3X(H ^`ynP3 Q,hÀ r2Bݡ_-2#bPkV$:DyQ(G(So g _SˬMċCͫ*?*ۥd88fWe=ss77:?cR )؞ ZԕrB^Z f}C:[+ܞCyd{S%,R_YocLY}in@/Tr)Txg{BR*zEW=(ZKOA}NH;"Q ܳ]dT;!lPڎhux52ߧEt[?:Q|Nt,6N~|Ɛg'tLHbU& >.+dxM![%RAhxR9pK|=I~-{T2L&Sy,Z3sHݲE1lmҷa5J`{T،q'WrYbzP:|Ou(EFRLr- 59O_d删j:.5NiyG$N1V'1iPƒpLzB[(M% %CRvyWE$ ^ۢWg+z0b%hD#hC2^=k0B|K}6Z|/24 chϲD_ X-- ^#8AS*u"#\^ F7MnS-Uȃ)&C3Gʆܚhh.w\"_B]HQox&ȕrJ\L]eT~0 Ŏaѻd Y AH`|ADdH 4`K–8Eϧ~W_FE+O;>qm[OȾM7ִk`!Ubg.7*/u}۽ty$ʊUNA֖gzjEy$e%Ǎx導w,d1W"愩0T&"r *AlV6-;<)D7޽ix;'_[-̺4ՂBEhZ"|K .t#[ܲ O]y0ΐp)X$XNVu2Mq;9@IǠ.+ w;xݖG ʼnNc/&&/תuҨW=qMF_ǽJbL1IyA8cqГ'@88!Q qaޢ=` m?_@>zj;11l]wi\6 FB:%Vhǭ@ŎB)p8E< Ϭ-PðM<_IVbwu<Sʥt@f;Ce8IJ Z4B\YAFtXˍ݈'`g [r=ENL1u!/5m jIɟy `dP =$ &5ZF2[XDdͯ&/-:Qۡ<}drË99,bdXg$ɚx4:KZHU'+2]rko2~}'DY.;ꄛn6qӴ^ݩVjݨ}ӯTjOnZ~7udue$6.q/> fPmv2LLeg䙗lu$~ZSճje^٪T&|vωnNbMɷCYx'^BkTB=!WozIaך]k{a=pZ$}373x7]ÝCpBbTnsƜ\T1 nnUp;ګ(P.bJL 9hx|%d]''xdCrِn!D7hȆbĒE䒊\6,faK˒ՋE7Di;/1x׾?g