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Waclaw Szybalski, D.Sc.

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W. Szybalski photo Professor Emeritus of Oncology

Ch.Eng., 1944, Chemistry, Institute of Technology, Lwów, Poland
M.Ch.Eng., 1945, Chemical Engineering, Institute of Technology, Gliwice, Poland
D.Sc., 1949, Microbial Biochemistry, Institute of Technology, Gdansk, Poland

Office: 618A McArdle Laboratory
Telephone:
Office - (608) 262-1259; Lab - (608) 262-2047
Email: szybalski@oncology.wisc.edu

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Research Interests: Novel methods for genome mapping and sequencing

Research Description: The objectives of our research on various aspects of genomics, including gene cloning, architecture and expression have been:
 (1) developing new methods for (i) ordering and in situ amplification of 30- to 100-kb fragments of large eukaryotic genomes, without conventional cloning, and (ii) ordered and automated sequencing of such large fragments by employing our SPEL-6 technique, based on primer assembly by hexamer ligation and primer walking;
(2) developing improved derivatives of pBAC vectors allowing conditional in vivo excision and amplification of 3- to 10-kb (cDNA) or 30- to 200 kb (genomic DNA) inserts in E. coli hosts [for more details, please see this abstract];
(3) the design and construction of novel regulatory circuits;
(4) the cloning of viral genes with dominant-lethal mutations as the basis for developing a new kind of immunity control against viral infection of plants and other organisms; and
(5) modifying and increasing specificity of restriction enzymes.

Selected recent publications

Szybalski, W.  The 50th Anniversary of Gene Therapy:  Beginnings and Present Realities.  Gene, 525(2): 151-154, 2013. 

Lobocka, M., and Szybalski, W. T.  Bacteriophages, Part A. Preface.  Adv. Virus Res., 82:  xiii-xv, 2012.

Lobocka, M., and Szybalski, W. T.  Phages.  Preface.  Adv. Virus Res., 83:  xvii-xviii, 2012.

Malecki, M., and Szybalski, W.  Isolation of Single, Intact Chromosomes from Single, Selected Ovarian Cancer Cells for in Situ Hybridization and Sequencing.  Gene, 493: 132-139, 2012.

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A complete list of Publications is available.